Analysis of glycosphingolipids from human cerebrospinal fluid

By David Priestman, PhD, Maria Leondaraki, Danielle Taylor-te Vruchte, BSc, Kerri-Lee Wallom, Maria E Fernandez-Suarez and Frances Platt, PhD
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Output Details

Description

We have updated and refined the GSL analysis protocol with the focus on achieving sensitive and reproducible quantitation of GSLs in control and patient cerebrospinal fluid samples. The method uses the fluorescent compound anthranilic acid (2-AA) to label oligosaccharides prior to analysis using normal-phase high-performance liquid chromatography. The labelling procedure is rapid, selective, and easy to perform. With the inclusion of a 2AA-labelled chitotriose calibration standard, it is possible to obtain accurate and reproducible molar quantities of individual GSL species.

Identifier (DOI)
10.17504/protocols.io.5qpvorrndv4o/v1
Tags
  • Glucosyl Ceramide
  • Glycosphingolipids
  • HPLC (High performance liquid chromatography)
  • Oligosaccharide Analysis
  • Other
  • Parkinson's disease
Team
Team Hardy
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • David Priestman, PhD

    Key Personnel: Team Hardy, 

    University of Oxford

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    Maria Leondaraki

  • Danielle Taylor-te Vruchte, BSc

    Key Personnel: Team Hardy, 

    University of Oxford

  • User avatar fallback logo

    Kerri-Lee Wallom

  • User avatar fallback logo

    Maria E Fernandez-Suarez

  • Frances Platt, PhD

    Co-PI (Core Leadership): Team Hardy, 

    University of Oxford

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Aligning Science Across Parkinson's
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