Mutations in mitochondrial protein CHCHD2 lead to a form of Parkinson's disease. CHCHD2 T61I mutant mice show molecular changes in the brain, altered metabolism favoring glycolysis, and disrupted mitochondrial function in dopamine neurons.
This protocol outlines steps for performing ex vivo slice whole-cell patch-clamp electrophysiology experiments, including optogenetic stimulation introduced through viral injection or transgenic expression.
