ATP-bound human mtHsp60:mtHsp10 half-football complex (C7), SPA reconstruction
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Description
Using cryo–electron tomography in human cells, we investigated the structural response of mitochondria to proteostatic stress. Stress induced protein aggregation in the mitochondrial matrix, remodeling of cristae architecture, and a reduction in mitochondrial ribosome complexes, while mitochondrial Hsp60 adopted conformations favoring complexes with its co-chaperone Hsp10 and interacting with native substrates. Complementary high-resolution single-particle cryo-EM reconstructions of mHsp60 provided mechanistic insight into its nucleotide-dependent functional cycle.
