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Chromium single-nucleus RNA Sequencing using the 10x Genomics Chromium Platform

By Marta Graziano, Ioannis Mantas, Konstantinos (Dinos) Meletis, PhD
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Output Details

Description
This protocol describes the isolation, fluorescence-activated sorting, and sequencing of nuclei from mouse striatal tissue for single-nucleus RNA sequencing (snRNA-seq) using the 10x Genomics Chromium Next GEM Single Cell 3′ Gene Expression platform. The workflow includes nuclei isolation, fluorescence-activated nuclei sorting (FANS), library preparation, and high-throughput sequencing for downstream transcriptomic analysis.
Identifier (DOI)
10.17504/protocols.io.5jyl8qznrl2w/v1
Discovery Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

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Datasets and Key Resources Table used in Qiao, Q. et al. (2026) 5-HT4 Receptor Ligand RS67333 Modulates Striatal Acetylcholine and Dopamine Release via the Inhibition of Acetylcholinesterase

This repository contains: 1) Key Resources Table containing details on key resources and the persistent identifiers for protocols and code used and generated in this study; 2) Source Data; and 3) README file.

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