DAB Immunohistochemistry (IHC) Staining for Stereological Analysis

DAB (3,3'-diaminobenzidine) is oxidized in the presence of peroxidase and hydrogen peroxide resulting in the deposition of a brown, alcohol-insoluble precipitate at the site of enzymatic activity.DAB (3, 3'-diaminobenzidine) produces a dark brown reaction product and can be used for immunohistochemical and applications. This protocol details the DAB immunohistochemistry staining for stereological analysis of 40 µM slices cut with cryostat and stored in antifreeze.