Data Acquisition and Analysis of Derivatized Hexose/Glucose by LC-QQQ-MS

This protocol outlines the steps for the separation, detection, and quantification of derivatized hexose/glucose using LC-QQQ-MS. Samples are analyzed with a C18 column coupled to a 1290 II LC system and an Ultivo triple quadrupole mass spectrometer. The procedure includes chromatographic conditions, MS settings, and data analysis workflows for high-throughput metabolite quantification.