Design and preparation of synthetic reference peptides for APP/Aβ TOMAHAQ proteomics, version 2

By Hankum Park, Frances Hundley, PhD and J. Wade Harper
View Published Protocol

Output Details

Description

This protocol describes the design and preparation of synthetic reference peptides for APP/Aβ TOMAHAQ proteomics

Identifier (DOI)
10.17504/protocols.io.bp2l6bqk1gqe/v2
Tags
  • Endolysosomes
  • Lysosomes
  • Proteomics
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

Related Research

Spatial snapshots of amyloid precursor protein intramembrane processing via early endosome proteomics

The authors developed an assay, Endo-IP, to rapidly isolate early and sorting endosomes. Using this method, they found a unique proteomic landscape of early/sorting endosomes, distinct from lysosomal proteomic landscape.

View Article

Urinary bis(monacylglycerol) phosphate (BMP) levels are higher in LRRK2 and GBA1 variant carriers but do not predict disease progression in PPMI cohorts

We quantified concentrations of three isoforms of the endolysosomal lipid, bis(monoacylglycerol) phosphate (BMP) in urine of deeply phenotyped cohorts in the Parkinson’s Progression Markers Initiative: LRRK2 G2019S PD (N = 134) and…

View Article

Early Endosome Capture Proteomics and its Application to Amyloid Precursor Protein Intramembrane Processing by β and γ-Secretases

Degradation and recycling of plasma membrane proteins occur through the endolysosomal system. Endo-IP, captures early endosomes for proteomic analysis, revealing insights into protein trafficking and disease-related cargo like APP in Alzheimer's.

View Article
View More Outputs