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Endogenous tagging of the YIPF4 gene with mNEON Green and imaging of these cells by microscopy
Output Details
Description
This protocol describes a method to create cells in which the YIPF4 gene has been endogenously tagged with mNEON fluorescent protein. CRISPR-based gene editing was used to introduce mNEON Green into the N-terminus of YIPF4 in WT and FIP200-/- HEK293T cells. We also describe a protocol for imaging of mNEON-YIPF4 in Golgi using the Golgi marker GOLGB1.
Identifier (DOI)
10.17504/protocols.io.5jyl8pj9dg2w/v1