Ex Vivo Mouse Brain Slice Whole-Cell Patch-Clamp Electrophysiology
Output Details
Description
This protocol outlines steps for performing ex vivo slice whole-cell patch-clamp electrophysiology experiments, including voltage clamp and current clamp recordings as well as optogenetic stimulation in the Ding lab. For optogenetic stimulation, this procedure is typically performed on mice expressing genetically encoded opsins, such as Channelrhodopsin2, which can be introduced through viral injection or transgenic expression.
Identifier (DOI)
10.17504/protocols.io.kqdg3q56pv25/v1