Ex Vivo Mouse Brain Slice Whole-Cell Patch-Clamp Electrophysiology

Output Details

This protocol outlines steps for performing ex vivo slice whole-cell patch-clamp electrophysiology experiments, including voltage clamp and current clamp recordings as well as optogenetic stimulation in the Ding lab. For optogenetic stimulation, this procedure is typically performed on mice expressing genetically encoded opsins, such as Channelrhodopsin2, which can be introduced through viral injection or transgenic expression.
Tags
  • Activity Assay
  • Electrophysiology
  • Ex Vivo
  • Patch-clamp

Meet the Authors

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    Fuu Jiun Hwang, PhD

    Key Personnel: Team Surmeier

    Stanford University

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    Jun Ding, PhD

    Collaborating PI: Team Surmeier

    Stanford University