Extracellular Markers Staining Protocol for Flow Cytometry

Research Community
Research Community

ASAP supports three programs and serves as a member of multiple consortia to advance research for the benefit of the global Parkinson’s disease community.
Explore
Highlights
Highlights

ASAP shares the impact that our initiative, network, and supported programs have had on providing new insights into Parkinson’s disease. Discover news, interviews, awards, and annual impact.
Explore
Open Science
Open Science

ASAP is dedicated to facilitating a research environment where meaningful collaboration, research-enabling resources, and data sharing provides the answers we need to understand, diagnose, and treat Parkinson’s disease. Discover our open science philosophy.
Explore
Tools & Resources
Tools & Resources

ASAP is committed to developing and openly sharing research outputs, including data, code, protocols, and key lab materials, to help the scientific community build upon existing work.
Explore

Description

This protocol describes the standard procedure for staining cell surface antigens on single-cell suspensions from mouse spleen, lymph nodes, and/or thymus for flow cytometric analysis.

Identifier (DOI)

10.17504/protocols.io.q26g7qykklwz/v1

Tags

Flow cytometry
Other

Discovery Team

Team Desjardins

Program

Collaborative Research Network

Theme

Neuro-Immune Interactions

Meet the Authors

Moustafa Elemeery

External Collaborator

Extracellular Markers Staining Protocol for Flow Cytometry

Output Details

Meet the Authors

Moustafa Elemeery

Related Research

Adoptive transfer of mitochondrial antigen-specific CD8+ T-cells in mice causes parkinsonism and compromises the dopamine system

T cell purification and activation

Intracellular Cytokine Staining (ICS) Protocol for FACS Analysis