Fiber Photometry (Mouse)

By Alexandra Nelson, MD, PhD
View Published Protocol

Output Details

Description

This protocol describes the procedure for fiber photometry in awake behaving mice. It includes details on the surgical implantation of fibers.

Identifier (DOI)
10.17504/protocols.io.b9udr6s6
Tags
  • Animal
  • Calcium
  • In Vivo
  • Live-imaging
  • Mouse
Team
Team Edwards
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

Related Research

Photometry Analysis Code

This set of functions extracts GCaMP Fiber Photometry data obtained with a TDT system, performs isosbestic and exponential correction, and computes the number and amplitude of transients over time. The code was originally used for the following…

View Code

Performing surgeries, Image processing and Analysis on Animals

This protocol details the experimental procedures on animals that includes the surgery, two-photon imaging, cued lever-pushing task and analysis includes movement behavior analysis, and PCA.

View Protocol

Stereotactic injection of viral vectors

Here we describe stereotactic injection of AAV into ventral tegmental area (VTA) of mice.

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.