Co-PI (Core Leadership): Team Schapira,
Institut Imagine
Key Personnel: Team Schapira,
Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)
Mutations in GBA1, the gene encoding the lysosomal enzyme β-glucocerebrosidase (GCase), which cause Gaucher’s disease, are the most frequent genetic risk factor for Parkinson’s disease (PD). Here, we employ global proteomic and single-cell…
Protocol for adding a 3X HA tag to TMEM192 gene in human cells using CAS9 and recombination template is described for H9 ES AAVS1-NGN2 cells.
Method that allows the staining of intact Golgi using GolgiTracker for subsequent flow cytometry analysis.
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