Flow cytometry-based measurement of trafficking of Golgi to the lysosome via autophagy - Aligning Science Across Parkinson's

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Description

Protocol for flow cytometry-based measurement of Golgi trafficking to lysosomes via autophagy using Keima-YIPF3/4 reporters in HEK293 cells.

Identifier (DOI)

10.17504/protocols.io.n92ldmpznl5b/v1

Tags

Autophagy
Flow cytometry

Discovery Team

Team Harper

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

J. Wade Harper

Lead PI (Core Leadership): Team Harper,

Harvard University
Sharan Swarup

External Collaborator
Kelsey Hickey

External Collaborator