Fluorescence microscopy analysis of PINK1 localization upon GTPP treatment
Output Details
Description
Using cryo–electron tomography in human cells, we investigated the structural response of mitochondria to proteostatic stress. Stress induced protein aggregation in the mitochondrial matrix, remodeling of cristae architecture, and a reduction in mitochondrial ribosome complexes, while mitochondrial Hsp60 adopted conformations favoring complexes with its co-chaperone Hsp10 and interacting with native substrates. Complementary high-resolution single-particle cryo-EM reconstructions of mHsp60 provided mechanistic insight into its nucleotide-dependent functional cycle.
Identifier (DOI)
10.5281/zenodo.17699821
