Generation of knockout iPSCs using CRISPR-Cas9 genome editing

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Description

This protocol describes the genetic modification of induced pluripotent cells (iPSCs) using CRISPR-Cas9, including synthesis of gRNA plasmids, transfection, selection of clones, and sequencing of genomic DNA to confirm knockout generation. The steps described in this protocol is based on Skarnes et al. (2019) and Fernandopulle et al. (2018).

Identifier (DOI)

10.17504/protocols.io.36wgqnr33gk5/v1

Team

Team De Camilli

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Nisha Mohd Rafiq, PhD

Key Personnel: Team De Camilli,

Yale University
Pietro De Camilli, PhD

Lead PI (Core Leadership): Team De Camilli,

Yale University

Generation of knockout iPSCs using CRISPR-Cas9 genome editing

Output Details

Meet the Authors

Nisha Mohd Rafiq, PhD

Pietro De Camilli, PhD

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