Generation of SLC45A1 Knockout iPSC Line (WTC11-Ngn2 Line)

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Description

This protocol describes the generation of an SLC45A1 knockout ( or other any gene) in WTC11-Ngn2 induced pluripotent stem cells (iPSCs) by targeting the coding sequence (CDS) using the CRISPR-spCas9 system.

Identifier (DOI)

10.17504/protocols.io.e6nvw45zzlmk/v1

Discovery Team

Team Alessi

Program

Collaborative Research Network

Theme

PD Functional Genomics

Generation of SLC45A1 Knockout iPSC Line (WTC11-Ngn2 Line)

Output Details

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