Aligning Science Across Parkinson's Logo Text

Generation of SLC45A1 Knockout iPSC Line (WTC11-Ngn2 Line)

View Published Protocol

Output Details

Description
This protocol describes the generation of an SLC45A1 knockout ( or other any gene) in WTC11-Ngn2 induced pluripotent stem cells (iPSCs) by targeting the coding sequence (CDS) using the CRISPR-spCas9 system.
Identifier (DOI)
10.17504/protocols.io.e6nvw45zzlmk/v1
Discovery Team
Team Alessi
Program
Collaborative Research Network
Theme
PD Functional Genomics

Related Research

Release Notes – ASAP CRN Cloud – Version 4.0.0

ASAP CRN Cloud released version 4.0.0 with expanded datasets including Human Postmortem-derived Brain Sequencing and Mouse datasets. New collections and individual datasets were added, enhancing PMDBS and Mouse RNA sequencing data.

View Dataset

Tabular data from Mitochondrial Proteostasis Cryo-ET Analysis

Tabular output data from the Mitochondrial Proteostasis Cryo-ET Analysis as demonstrated in Ehses et al., 2026.

View Dataset

Biochemical analysis of PINK1 activation and ubiquitination signaling upon GTPP treatment

Ubiquitinated proteins were enriched using Halo-m-DSK ubiquitin capture, and GFP-PINK1 complexes were isolated by IP, followed by SDS–PAGE and immunoblotting to assess PINK1 activation and downstream ubiquitination signaling upon GTPP treatment

View Dataset
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.