Aligning Science Across Parkinson's Logo Text

Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism – ASAP protocol collection

By Michela Deleidi, MD, PhD, Pascale Baden, Maria Jose Perez Jimenez, Hariam Raji, Federico Bertoli
View Published Protocol

Output Details

Description
Collection of protocols of Deleidi Lab used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism"
Identifier (DOI)
10.17504/protocols.io.x54v9d44mg3e/v1
Tags
  • Activity Assay
  • GCase activity
  • Immunofluorescence
  • Immunoprecipitation
  • Lentivirus
  • Western Blot
Discovery Team
Team Schapira
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

Meet the Authors

  • Michela Deleidi, MD, PhD

    Co-PI (Core Leadership): Team Schapira, 

    Institut Imagine

  • User avatar fallback logo

    Pascale Baden

    External Collaborator

  • User avatar fallback logo

    Maria Jose Perez Jimenez

    External Collaborator

  • User avatar fallback logo

    Hariam Raji

    External Collaborator

  • User avatar fallback logo

    Federico Bertoli

    External Collaborator

Related Research

Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism

Mutations in GBA1, the gene encoding the lysosomal enzyme β-glucocerebrosidase (GCase), which cause Gaucher’s disease, are the most frequent genetic risk factor for Parkinson’s disease (PD). Here, we employ global proteomic and single-cell…

View Article

CoxII degradation assay to assess mitophagy

This protocol details the procedure to assess mitophagy by analyzing COXII degradation via Western blotting.

View Protocol

Complex I activity assay

This protocol is part of a Collection of protocols (dx.doi.org/10.17504/protocols.io.8epv593dng1b/v1) for the paper "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly…

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.