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H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; CCPG1-/-; PiggyBac-Keima-REEP5
Output Details
Description
ES cells for making iNeurons lacking the ER-phagy receptor FAM134C,A,B, TEX264, CCPG1 and expressing the indicated Keima-REEP5 ER-phagy flux reporter
PubMed=37425907;
Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2
construct was introduced in the AAVS1 safe harbor locus (from parent
cell line).
Characteristics: Transduced with a PiggyBac transposon vector
pAC150-PBLHL-4xHS-EF1a-Keima-REEP5 that localizes specifically to
plasmic reticulum tubules (PubMed=37425907).
Knockout cell: Method=CRISPR/Cas9; 24227; CCPG1.
Knockout cell: Method=CRISPR/Cas9; 25964; RETREG1.
Knockout cell: Method=CRISPR/Cas9; 28450; RETREG2.
Knockout cell: Method=CRISPR/Cas9; 27258; RETREG3.
Knockout cell: Method=CRISPR/Cas9; 30247; TEX264.
Transfected with: HGNC; 13805; NEUROG2.
Transfected with: HGNC; 30077; REEP5.
Transfected with: UniProtKB; Q1JV70; Montipora sp. 20 fluorescent
protein mKeima.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
NCBI_TaxID=9606; ! Homo sapiens (Human)
RRID:CVCL_D1IE ! H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/-
64-/- CCPG1-/-
Female
Blastocyst stage
Embryonic stem cell