Hybrid selection protocol using 10x Single-Cell RNA-Seq assay library

Description

This protocol is for target enrichment of cDNA libraries generated with 10x Genomics single-cell RNA-seq assays. The protocol consists of parts of vendor provided protocols with minor modification. The hybridization and PCR part is based on "CG000059_DemonstratedProtocolExome_RevC" (10x Genomics) and the capture part is based on "SureSelectXT Target Enrichment System for Illumina Paired-End Multiplexed Sequencing Library Protocol Version C3, September 2019" (Agilent).

Identifier (DOI)

10.17504/protocols.io.x54v924jzl3e/v1

Tags

10X Genomics
Human
Postmortem

Discovery Team

Team Scherzer

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Xian Adiconis

External Collaborator
Joshua Levin, PhD

Co-PI (Core Leadership): Team Scherzer,

Broad Institute

Hybrid selection protocol using 10x Single-Cell RNA-Seq assay library

Output Details

Meet the Authors

Xian Adiconis

Joshua Levin, PhD

Related Research

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