Identification of phosphorylation sites on NAP1/AZI2

By Dr WeiQiang Chen and Sascha Martens
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Output Details

Description
We observed smearing for the TBK1-adaptor protein NAP1/AZI2 during mitophagy. To verify whether this smearing was due to phosphorylation by TBK1, we performed an in vitro kinase assay and complemented this with the immunoprecipitation of NAP1 from HAP1, whose genetic background (FIP200 knockout) results in hyperactivation of TBK1.
Tags
  • Mass spectrometry
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

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