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Immunofluorescence for tyrosine hydroxylase (TH) in mouse brain sections

By Marta Graziano, Ioannis Mantas, Konstantinos (Dinos) Meletis, PhD
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Output Details

Description
This protocol describes the immunohistochemical staining procedure for detecting Tyrosine Hydroxylase (TH) in mouse brain sections using primary antibodies and fluorescent-conjugated secondary antibodies. This protocol ensures optimal signal specificity and preservation of tissue morphology.
Identifier (DOI)
10.17504/protocols.io.gznqbx5dx
Discovery Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

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Datasets and Key Resources Table used in Qiao, Q. et al. (2026) 5-HT4 Receptor Ligand RS67333 Modulates Striatal Acetylcholine and Dopamine Release via the Inhibition of Acetylcholinesterase

This repository contains: 1) Key Resources Table containing details on key resources and the persistent identifiers for protocols and code used and generated in this study; 2) Source Data; and 3) README file.

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