Immunohistology in mouse brain sections after whole-brain micro-CT scanning

Output Details

Howe's Lab developed a new micro-fiber array approach capable of chronically measuring and optogenetically manipulating local dynamics across over 100 targeted locations simultaneously in head-fixed and freely moving mice, enabling investigation of cell-type and neurotransmitter-specific signals over arbitrary 3-D volumes. This protocol includes the immunohistology steps used to confirm viral expression, and/or stain for neurons after micro-CT scanning and contrast agent soaking procedure.
Tags
  • Antibodies
  • Confocal microscopy
  • Dopaminergic neurons
  • Microscopy - optical
  • Mouse
  • Viral vectors

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