Immunological detection of APP and proteins of the endolysosomal system

By Hankum Park, PhD, Frances Hundley, PhD and J. Wade Harper
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Output Details

Description

This protocol describes the immunological detection of APP and proteins of the endolysosomal system.

Here we present a general protocol for immunological detection by Western blotting of APP and proteins of the endolysosomal system, including EEA1, RAB5, PSEN1, LAMP1, LAMP2, TMEM192, and BACE1.

 

 

Identifier (DOI)
10.17504/protocols.io.kqdg36jeeg25/v2
Tags
  • Endolysosomes
  • Endosomes
  • Lysosomes
  • Western Blot
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

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