Immunostaining Mouse Brain Tissue or Neuronal Cultures

By Shweta Jain, PhD and Robert Edwards, MD
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Output Details

Description
This protocol describes immunohistochemical and immunoctyochemical methods to analyze protein expression in mouse brain. In the protocol, we describe the fixation of tissue or cultured neurons, the immunostaining procedure, and collecting images for analysis.
Identifier (DOI)
10.17504/protocols.io.ewov1qow7gr2/v1
Tags
  • Brain
  • Ex Vivo
  • Immunohistochemistry
  • Microscopy - optical
  • Mouse
  • Primary culture
Team
Team Edwards
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

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