In vitro kinase assay: phosphorylation of PI3Kc1 by TBK1and ULK1

By Elias Adriaenssens, PhD
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Output Details

Description
This protocol describes in vitro kinase assay, in which PI3Kc is tested for phosphorylation by TBK1 and ULK1 complex either by western blotting (S29 on ATG14L subunit) or mass spectrometry analysis.
Identifier (DOI)
10.17504/protocols.io.j8nlkwr51l5r/v1
Tags
  • Kinase
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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    Elias Adriaenssens, PhD

    Key Personnel: Team Hurley, 

    Max F. Perutz Laboratories

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