L1-silenced iPSC line (HS1_L1HS-CRISPRi_g1)

By Anita Adami, MSc and Johan Jakobsson, PhD
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Output Details

Description
We generated a collection of human iPSCs transduced with a CRISPRi construct targeting evolutionary young LINE-1 retrotransposons. The stably transduced lines include two different genomic backgroung (HS1 & HS2), two different guide RNAs (g1 & g2) and the appropriate non-targeting control (LacZ). - **HS1_L1HS-CRISPRi_g1 (RRID:CVCL_E8I4)** - HS1_L1HS-CRISPRi_g2 (RRID:CVCL_E8I5) - HS1_L1HS-CRISPRi_LacZ (RRID:CVCL_E8I6) - HS2_L1HS-CRISPRi_g1 (RRID:CVCL_E8I7) - HS2_L1HS-CRISPRi_LacZ (RRID:CVCL_E8I8) These cell lines are published a lab resource generated as part of the work included in Adami et al. ([10.1016/j.xgen.2025.100979 ](https://doi.org/10.1016/j.xgen.2025.100979))
Tags
  • CRISPRi dCas9
  • hiPSCs (Human induced pluripotent stem cells)
  • Human
  • Transposable elements
Team
Team Jakobsson
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

Meet the Authors

Related Research

LINE-1 retrotransposons regulate the exit of human pluripotency and early brain development (Preprint)

Hominoid-specific L1 retrotransposons are expressed in human stem cells and organoids. Silencing L1s leads to changes in neural differentiation and organoid size, suggesting L1 involvement in central nervous system development.

View Article

L1-silenced iPSC line (HS1_L1HS-CRISPRi_g2 )

Human iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).

View Lab Material

L1-silenced iPSC line – Control (HS1_L1HS-CRISPRi_LacZ )

Human iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).

View Lab Material
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