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Live-cell imaging; mitochondrial reactive oxygen species
Output Details
Description
This protocol presents instructions for assessing mitochondrial souced reactive oxygen species using MitoTracker® Red CM-H2XRos dye (Thermo Fisher Scientific), a reduced non-fluorescent version of Mitotracker red that is fluorescent upon oxidation within mitochondria, which accumulates in mitochondria upon oxidation.
Identifier (DOI)
10.1073/pnas.0904764106