Measurement of Lysosomal pH Using LysoSensor Yellow/Blue DND-160

Research Community
Research Community

ASAP supports three programs and serves as a member of multiple consortia to advance research for the benefit of the global Parkinson’s disease community.
Explore
Highlights
Highlights

ASAP shares the impact that our initiative, network, and supported programs have had on providing new insights into Parkinson’s disease. Discover news, interviews, awards, and annual impact.
Explore
Open Science
Open Science

ASAP is dedicated to facilitating a research environment where meaningful collaboration, research-enabling resources, and data sharing provides the answers we need to understand, diagnose, and treat Parkinson’s disease. Discover our open science philosophy.
Explore
Tools & Resources
Tools & Resources

ASAP is committed to developing and openly sharing research outputs, including data, code, protocols, and key lab materials, to help the scientific community build upon existing work.
Explore

Description

This protocol describes the measurement of lysosomal pH using the ratiometric dye LysoSensor Yellow/Blue DND-160. The method includes dye incubation, fluorescence measurement, and the use of a pH calibration curve to derive quantitative lysosomal pH values.

Identifier (DOI)

10.17504/protocols.io.q26g7nrx1lwz/v1

Discovery Team

Team Alessi

Program

Collaborative Research Network

Theme

PD Functional Genomics

Measurement of Lysosomal pH Using LysoSensor Yellow/Blue DND-160

Output Details

Related Research

Release Notes – ASAP CRN Cloud – Version 4.0.0

Tabular data from Mitochondrial Proteostasis Cryo-ET Analysis

Biochemical analysis of PINK1 activation and ubiquitination signaling upon GTPP treatment