Measurement of Lysosomal pH Using LysoSensor Yellow/Blue DND-160

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Description

This protocol describes the measurement of lysosomal pH using the ratiometric dye LysoSensor Yellow/Blue DND-160. The method includes dye incubation, fluorescence measurement, and the use of a pH calibration curve to derive quantitative lysosomal pH values.

Identifier (DOI)

10.17504/protocols.io.q26g7nrx1lwz/v1

Discovery Team

Team Alessi

Program

Collaborative Research Network

Theme

PD Functional Genomics

Measurement of Lysosomal pH Using LysoSensor Yellow/Blue DND-160

Output Details

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