Multicolor fluorescence in situ hybridization and analysis

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Description

Antipsychotics are known to induce the expression of several immediate early genes (IEGs) via D2R antagonism, which then leads to changes in critical signaling pathways in iSPNs. To define the impact of LRRK2 kinase activity on the expression pattern of IEGs in the iSPNs, we used multicolored single-molecule fluorescence in situ hybridization.

Identifier (DOI)

10.17504/protocols.io.3byl4937rgo5/v1

Discovery Team

Team Awatramani

Program

Collaborative Research Network

Theme

Circuitry and Brain-Body Interactions

Meet the Authors

Chuyu Chen

External Collaborator
Loukia Parisiadou, PhD

Co-PI (Core Leadership): Team Awatramani,

Northwestern University (Chicago)

Multicolor fluorescence in situ hybridization and analysis

Output Details

Meet the Authors

Chuyu Chen

Loukia Parisiadou, PhD

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