Multiscale model of primary motor cortex circuits predicts in vivo cell type-specific, behavioral state-dependent dynamics

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Published June 9, 2023

Understanding cortical function requires studying multiple scales: molecular, cellular, circuit and behavior. We developed a biophysically detailed multiscale model of mouse primary motor cortex (M1) with over 10,000 neurons and 30 million synapses. Neuron types, densities, spatial distributions, morphologies, biophysics, connectivity and dendritic synapse locations were tightly constrained by experimental data. The model includes long-range inputs from 7 thalamic and cortical regions, as well as noradrenergic inputs from locus coeruleus. Connectivity depended on cell class and cortical depth at sublaminar resolution. The model accurately predicted in vivo layer- and cell type-specific responses (firing rates and LFP) associated with behavioral states (quiet wakefulness and movement) and experimental manipulations (noradrenaline receptor blocking and thalamus inactivation). It also enabled evaluation of multiple mechanistic hypotheses underlying the observed activity. This quantitative theoretical framework can be used to integrate and interpret M1 experimental data and sheds light on the cell type-specific multiscale dynamics associated with a range of experimental conditions and behaviors.
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  • Original Research

Meet the Authors

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    Salvador Dura-Bernal

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    Samuel A. Neymotin

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    Benjamin A. Suter

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    Joshua Dacre

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    Joao V.S. Moreira

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    Eugenio Urdapillete

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    Julia schiemann

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    Ian Duguid

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    Gordon M.G. shepherd

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    William Lytton, MD

    Collaborating PI: Team Wichmann

    SUNY Downstate