Neuromelanin quantification in stained brain slices of the Locus coeruleus

Description

This protocol describes an automatizable pipeline for cell counting based on fluorescent stainings with the help of CellPose. Additionally, it describes the entire generation process of plots that show the intensity of neuromelanin per cell based on bright-field images. The protocol includes Fiji macro codes for data analysis and an R code for plot generation. This pipeline has been used for the analysis of locus coeruleus data.

Identifier (DOI)

10.17504/protocols.io.5qpvo32y9v4o/v1

Team

Team Vila

Program

Collaborative Research Network

Theme

Circuitry and Brain-Body Interactions

Meet the Authors

Csilla Novák, MSc

Key Personnel: Team Vila,

Leibniz Institute for Neurobiology
Maria Paz Contreras Santander, MD, PhD

Key Personnel: Team Vila,

Leibniz Institute for Neurobiology
Matthias Prigge, PhD

Co-PI (Core Leadership): Team Vila,

Leibniz Institute for Neurobiolog

Neuromelanin quantification in stained brain slices of the Locus coeruleus

Output Details

Meet the Authors

Csilla Novák, MSc

Maria Paz Contreras Santander, MD, PhD

Matthias Prigge, PhD

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