Step-by-step protocol for ONT direct RNA sequencing: RNA extraction, mRNA poly(A) selection, library preparation for ONT direct RNA sequencing, and ONT direct RNA data analysis.
The authors developed an iPSC toolbox utilizing piggyBac-based or targeted transgenes to rapidly induce CNS cells with concomitant expression of aggregation-prone proteins.
Step-by-step protocol for performing HyDrop-RNA. The duration of each step assumes an experienced protocol user. For a first-time user, we recommend doubling the expected time for each step.
