Passaging of hPSCs grown on MEFs

Output Details

This protocol describes the standard procedure of using collagenase to passage human pluripotenct stem cells (hPSCs) on inactivated mouse embryonic fibroblasts (MEFs).

Tags
  • hiPSCs (Human induced pluripotent stem cells)
  • hPSCs (Human pluripotent stem cells)

Meet the Authors

  • Oriol Busquets Figueras, PhD

    Key Personnel: Team Rio

    Albert Einstein College of Medicine

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    Hanqin Li, PhD

    Key Personnel: Team Rio

    University of California, Berkeley

  • Steven Poser, PhD

    Key Personnel: Team Rio

    University of Washington

  • Frank Soldner, MD

    Co-PI (Core Leadership): Team Rio

    Albert Einstein College of Medicine

  • Dirk Hockemeyer, PhD

    Co-PI (Core Leadership): Team Rio

    University of California, Berkeley