Passaging of hPSCs grown on MEFs

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Description

This protocol describes the standard procedure of using collagenase to passage human pluripotenct stem cells (hPSCs) on inactivated mouse embryonic fibroblasts (MEFs).

Identifier (DOI)

10.17504/protocols.io.b4mpqu5n

Tags

hiPSCs (Human induced pluripotent stem cells)
hPSCs (Human pluripotent stem cells)

Discovery Team

Team Rio

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Oriol Busquets Figueras

External Collaborator
Hanqin Li

External Collaborator
Steven Poser

External Collaborator
Frank Soldner, MD

Co-PI (Core Leadership): Team Rio,

Albert Einstein College of Medicine
Dirk Hockemeyer, PhD

Co-PI (Core Leadership): Team Rio,

University of California, Berkeley

Passaging of hPSCs grown on MEFs

Output Details

Meet the Authors

Oriol Busquets Figueras

Hanqin Li

Steven Poser

Frank Soldner, MD

Dirk Hockemeyer, PhD

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