Aligning Science Across Parkinson's Logo Text

Phosphatome-wide siRNA screen in 3T3 cells

View Published Protocol

Output Details

Description
This protocol describes how we performed a phosphatome-wide siRNA screen in 3T3 cells to look for LRRK2-counteracting phosphatases for pRab12 and pRab10.
Identifier (DOI)
10.17504/protocols.io.36wgqdxr5vk5/v1
Team
Team Alessi
Program
Collaborative Research Network
Theme
PD Functional Genomics

Related Research

ASAP CRN Cloud Release Notes – Version 4.0.0

ASAP CRN Cloud released version 4.0.0 with expanded datasets including Human Postmortem-derived Brain Sequencing and Mouse datasets. New harmonized collections and individual datasets were added, enhancing research possibilities.

View Code

An ImageJ/FIJI Preprocessing Workflow for Multi-Series Confocal Microscopy Datasets Prior To CellProfiler Analysis

A workflow using ImageJ/FIJI aims to create three-slice MIPs from multi-series .czi files to capture weak signals. It involves extracting datasets, creating projections, and saving images for efficient analysis in CellProfiler.

View Protocol

A CellProfiler Pipeline for Quantification of p-SNCA in Mouse Striatal Cholinergic and Medium Spiny Neurons

Brain sections stained for pSNCA, DARPP-32, ChAT, and DAPI. Z-stacks processed into MIPs for analysis. Outputs include pSNCA intensity and neuronal mask area, normalised for cell type-specific burden using R script.

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.