Mutations in ATP13A2 gene can cause familial Parkinson's disease. Deleting ATP13A2 in adult mice leads to dopaminergic nerve terminal loss and neuronal degeneration, mimicking symptoms of ATP13A2-related neurodegenerative diseases.
The authors present a method to identify the same brain region in human postmortem tissue to evaluate ciliary status. The striatum is identified morphologically and also by immunofluorescence microscopy using anti-DARPP-32 antibodies.
This is series of protocols that has been adapted from published and unpublished protocols broadly referred to as the QUINT workflow:
QuPath visualization/segmentation
QuickNII Brain Atlas Registration
QMask Hemispheric Separation
Visualign…
