Probedesign pipeline for the inhouse generation of seqFISH probes

Output Details

The probedesign pipeline works by inputting the transcript id's for which probes have to be designed. Additional probe design parameters such as GC% or melting temperature can be changed via the config file. The pipeline will output the designed probes in fasta format as well as a csv file with the results of all filtering steps.
Tags
  • Sequential fluorescence in situ hybridization (seqFISH)
  • Spatial transcriptomics

Meet the Authors

  • User avatar fallback logo

    Ceyhun Alar, MSc

    Key Personnel: Team Voet

    KU Leuven

  • Alejandro Sifrim, PhD

    Key Personnel: Team Voet

    KU Leuven