Rat organotypic cultures for AAV-mediated vital labeling of the extracellular matrix V.2

By Federico Soria, PhD and Mario Fernandez Ballester, MSc
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Output Details

Description
In this protocol we describe the preparation and maintenance of rat organotypic cultures (cortico-striatal) for AAV-infection and imaging of the extracellular matrix. The AAV, in this case, induces the expression of a exportable hyaluronan-binding protein (the HA-biding domain of Neurocan) fused with GFP, that allows vital labeling (and hence live imaging) of the extracellular matrix. The organotypic cultures can be maintained for several weeks, allowing for repeated imaging and longitudinal studies.
Identifier (DOI)
10.17504/protocols.io.e6nvwdnzzlmk/v2
Team
Team Vila
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

  • Federico Soria, PhD

    Collaborating PI: Team Vila, 

    Achucarro Basque Center for Neuroscience

  • Mario Fernandez Ballester, MSc

    Key Personnel: Team Vila, 

    University of the Basque Country

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