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Rat organotypic cultures for AAV-mediated vital labeling of the extracellular matrix V.2
Output Details
Description
In this protocol we describe the preparation and maintenance of rat organotypic cultures (cortico-striatal) for AAV-infection and imaging of the extracellular matrix. The AAV, in this case, induces the expression of a exportable hyaluronan-binding protein (the HA-biding domain of Neurocan) fused with GFP, that allows vital labeling (and hence live imaging) of the extracellular matrix. The organotypic cultures can be maintained for several weeks, allowing for repeated imaging and longitudinal studies.
Identifier (DOI)
10.17504/protocols.io.e6nvwdnzzlmk/v2