RNA Isolation Using QIAzol-RNeasy Mini Kit and cDNA Synthesis

This protocol outlines a streamlined workflow for isolating high-quality RNA using QIAzol and RNeasy Mini columns, followed by cDNA synthesis with the High-Capacity cDNA Reverse Transcription Kit. It covers cell harvesting, QIAzol lysis, phase separation, column purification, and RNA before reverse transcription. The method provides reproducible RNA recovery and reliable cDNA generation for downstream gene-expression applications.