Comparison of RNA QC run using either trimmomatic or fastp.
Provision of scripts for RNA-seq processing steps, from QC to alignment and quantification.
PD involves proteinopathy and astrogliosis. Oligomeric alpha-synuclein induces astrocyte inflammation, leading to neuronal toxicity. ADAR1-mediated RNA editing plays a role in sustaining inflammatory states, potentially driving PD neuroinflammation.
Protocol for dissection of midbrain from mice, followed by isolation of RNA from midbrain samples, reverse transcription of mRNA to cDNA, and quantitative real-time PCR for analysis of VMAT2 expression levels.
