Section 1: Enzymatic DNA Fragmentation (Manually)

By Ester Kalef-Ezra, PhD, Ben Harvey, Katherine Roper and Christos Proukakis, PhD
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Output Details

Description
This protocol details manual enzymatic DNA Fragmentation prior to Section 2: NGS library preparation for sequencing.
Identifier (DOI)
10.17504/protocols.io.kqdg3x3m7g25/v1
Tags
  • DNA
  • Human
Team
Team Voet
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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Section 2: NGS library preparation for sequencing

This protocol details NGS library preparation for sequencing and should be performed after Section 1: Enzymatic DNA Fragmentation (Manually).

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Section 3: Libraries quality control (QC)

This protocol details quality control of libraries and should be performed after Section 2: NGS library preparation for sequencing.

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SNP Genotyping and ApoE Genotyping

Protocol outlines DNA extraction from blood, quality control, SNP, and APOE genotyping. Adapted from PRoBaND SNP Genotyping and ApoE Genotyping Protocol by Malek et al. for Parkinson's Disease study.

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