Long-read RNA sequencing of SNCA in human dopaminergic neuron reveals most expression comes from alternative transcripts. Targeting these with ASOs effective reversal of PD pathology, offering insights for RNA therapies + protein isoform differences.
Using, highly sensitive ribosome-bound RNA sequencing and imaging to characterize the translatome, the authors uncovered local mRNA translation of dopamine synthesis, release, and reuptake machinery in dendrites, but not axons.
