Standard DAB Staining for Free-floating Fixed NHP Brain Tissue

This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex. This protocol has been tested with free-floating non-human primate (NHP) and rodent (mouse, rat) brain tissue that has been fixed (10% formalin or 4% paraformaldehyde), cryoprotected (sucrose or glycerol gradients), and cryo-sectioned (20 µm - 50 µm).