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Standard DAB Staining for Free-floating Fixed NHP Brain Tissue
Output Details
Description
This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex. This protocol has been tested with free-floating non-human primate (NHP) and rodent (mouse, rat) brain tissue that has been fixed (10% formalin or 4% paraformaldehyde), cryoprotected (sucrose or glycerol gradients), and cryo-sectioned (20 µm - 50 µm).
Identifier (DOI)
10.17504/protocols.io.261ged767v47/v2