Synapsin E-domain is essential for α-synuclein function

Output Details

Preprint June 27, 2023

Published February 26, 2024

The cytosolic proteins synucleins and synapsins are thought to play cooperative roles in regulating synaptic vesicle (SV) recycling, but mechanistic insight is lacking. Here we identify the synapsin E-domain as an essential functional binding-partner of α-synuclein (α-syn). Synapsin E-domain allows α-syn functionality, binds to α-syn, and is necessary and sufficient for enabling effects of α-syn at the synapse. Together with previous studies implicating the E-domain in clustering SVs, our experiments advocate a cooperative role for these two proteins in maintaining physiologic SV clusters.
Identifier (DOI)
10.7554/eLife.89687
Tags
  • Alpha-synuclein
  • Original Research
  • Protein-protein interaction
  • Synapse
  • Synaptic trafficking
  • Synaptic vesicle exocytosis

Meet the Authors

  • User avatar fallback logo

    Alexandra Stavsky

  • Leonardo Parra-Rivas, PhD

    Key Personnel: Team Gradinaru

    University of California, San Diego

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    Shani Tal

  • User avatar fallback logo

    Jen Riba

  • User avatar fallback logo

    Kayalvizhi Madhivanan

  • Subhojit Roy, MD, PhD

    Collaborating PI: Team Gradinaru

    University of California, San Diego

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    Daniel Gitler