Synapsin E-domain is essential for α-synuclein function

Description

The cytosolic proteins synucleins and synapsins are thought to play cooperative roles in regulating synaptic vesicle (SV) recycling, but mechanistic insight is lacking. Here we identify the synapsin E-domain as an essential functional binding-partner of α-synuclein (α-syn). Synapsin E-domain allows α-syn functionality, binds to α-syn, and is necessary and sufficient for enabling effects of α-syn at the synapse. Together with previous studies implicating the E-domain in clustering SVs, our experiments advocate a cooperative role for these two proteins in maintaining physiologic SV clusters.

Identifier (DOI)

10.7554/eLife.89687

Tags

Alpha-synuclein
Original Research
Protein-protein interaction
Synapse
Synaptic trafficking
Synaptic vesicle exocytosis

Team

Team Gradinaru

Program

Collaborative Research Network

Theme

Circuitry and Brain-Body Interactions

Meet the Authors

Alexandra Stavsky

External Collaborator
Leonardo Parra-Rivas, PhD

Key Personnel: Team Gradinaru,

University of California, San Diego
Shani Tal

External Collaborator
Jen Riba

External Collaborator
Kayalvizhi Madhivanan

External Collaborator
Subhojit Roy, MD, PhD

Collaborating PI: Team Gradinaru,

University of California, San Diego
Daniel Gitler

External Collaborator

Synapsin E-domain is essential for α-synuclein function

Output Details

Meet the Authors

Alexandra Stavsky

Leonardo Parra-Rivas, PhD

Shani Tal

Jen Riba

Kayalvizhi Madhivanan

Subhojit Roy, MD, PhD

Daniel Gitler

Related Research

Tabular data associated with “Synapsin E-domain is essential for α-synuclein function”

pHluorin assays, analysis, and fluorescence microscopy

Immunoprecipitations and Western blots analysis