Transcardiac Perfusion of Mouse for Brain Tissue

By Maryana Nissan, BSc and Divya Darwinarulseeli, PhD
View Published Protocol

Output Details

Description
Transcardiac perfusion is a method used for clearing blood and preserving the mouse brain for immunostaining.
Identifier (DOI)
10.17504/protocols.io.q26g7pzmkgwz/v1
Team
Team Awatramani
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

  • Maryana Nissan, BSc

    Key Personnel: Team Awatramani, 

    Northwestern University

  • Divya Darwinarulseeli, PhD

    Key Personnel: Team Awatramani, 

    Northwestern University

Related Research

L1-silenced iPSC line (HS1_L1HS-CRISPRi_g1)

Human iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).

View Lab Material

L1-silenced iPSC line (HS1_L1HS-CRISPRi_g2 )

Human iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).

View Lab Material

L1-silenced iPSC line – Control (HS1_L1HS-CRISPRi_LacZ )

Human iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).

View Lab Material
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.