Transfection of HEK cells with Lipofectamine 3000

View Published Protocol

Output Details

Description
Protocol used in the Gradinaru lab to transfect HEK cells with lipofectamine 3000
Identifier (DOI)
10.17504/protocols.io.6qpvr97wzvmk/v1
Tags
  • Cell culture
  • Transfection
Team
Team Gradinaru
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Related Research

Genetically encoded self-assembling synuclein for tunable Parkinsonian pathology in vitro and in vivo

This paper describes the self-assembling synuclein (SAS) system, a genetically-encoded tool for inducing temporally-controllable, tunable PD pathology in a wide range of in vitro and in vitro models.

View Article

fcs files to “The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model”

Dataset associated with DOI: 10.17617/3.51VXU0

View Dataset

GPNMB is a biomarker for lysosomal dysfunction and is secreted via LRRK2-modulated lysosomal exocytosis

*GPNMB* is linked to Parkinson's Disease as a risk factor, affecting its transcription and protein levels in CSF. Macrophages secrete GPNMB in response to lysosomal stress through lysosomal exocytosis, with LRRK2 influencing its secretion.

View Article
View More Outputs