Whole cell protein extraction and Western blotting

Description

This protocol describes cell lysis for whole-cell protein extraction followed by western blotting using LI-COR Odyssey quantitative western blot. Frozen cell pellets described here are typically iPSC-derived cortical neurons, seeded into a 24 well plate format at 1x10^6 cells per well, and collected for downstream processing at DIV25.

Identifier (DOI)

10.17504/protocols.io.rm7vzje8rlx1/v1

Team

Team Studer

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Isabel Lam, PhD

Key Personnel: Team Studer,

Brigham and Women's Hospital
Alain Ndayisaba, MD

Key Personnel: Team Studer,

Brigham and Women's Hospital
Vikram Khurana, MD

Co-PI (Core Leadership): Team Studer,

Harvard University

Whole cell protein extraction and Western blotting

Output Details

Meet the Authors

Isabel Lam, PhD

Alain Ndayisaba, MD

Vikram Khurana, MD

Related Research

Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of α-synuclein inclusions

Image Processing and 3D Reconstruction – Chen et al. 2025 NSMB

Plasmid construction – Chen et al. 2025 NSMB