Induction of starvation stress
By onProtocol describing the induction of starvation stress using EBSS in HeLa cells
Western blotting using the BioRad Criterion Blotter system
By onProtocol for performing an SDS-PAGE Western blot analysis using the BioRad Criterion Blotter system.
Analyzing cellular ATP levels
By onProtocol for analysis of cellular ATP levels using the Promega Mitochondrial ToxGlo Kit.
Transfection by electroporation of GFP-LRRK2 and Immunofluorescent imaging of MEFs VPS35 (D620N) mutants stably expressing LysoTag
By onThe authors investigated the colocalization of GFP-LRRK2 with a lysosomal localized TMEM192-3xHA.
In vitro depalmitoylation assay
By onThis protocol describes the in vitro depalmitoylation assay of DNAJC5.
Strep-pull down assay
By onStrep pull down assay for FIP200(NTD)-TSF WT or mutants were co-transfected with GST-ATG13(363-517) and/or ULK1(MIT)-MBP.
Synthesis of 1-(3-Chlorophenethyl)-3-cyclopentylpyrimidine-2,4,6-(1H,3H,5H)-trione
By on1-(3-chlorophenethyl)-3-cyclopentylpyrimidine-2,4,6-(1H,3H,5H)-trione (8) is a potent and highly selective Ca(V)1.3 L-type calcium channel antagonist. This protocol is an adaption of Kang et al. 2012 's method of the synthesis of this chemical.
Isolation of mCherry-LC3B and 97Q-GFP vesicles for LC-MS/MS
By onThe protocol outlines the isolation of mCherry-LC3B and 97Q-GFP vesicles for LC-MS/MS analysis, crucial for studying autophagy and protein aggregation.
Cytokine profiling analysis on conditioned medium of human neurons using Luminex multiplex assay
By onThis protocol is used to identify secreted inflammatory factors by cytokine profiling of the conditioned medium from human transdifferentiated neurons of healthy donors and AD patients.
Measuring alpha-synuclein concentration in hiPSCs using ELISA
By onThis protocol uses a classical ELISA experimental design with sensitive electrochemical detection to provide a measure of 𝜶-synuclein concentration in the conditioned media of cultured iPSC-derived human cells.
Detection of accessible cholesterol in primary cilia using purified His-ALOD4-mNeon in 3T3 Fibroblasts
By onThe protocol described here is based upon previously established methods (Kinnebrew et al., 2019; Johnson and Radhakrishnan, 2021) and has also been used successfully to label cilia in RPE cells.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices
By onThis protocol is to assess whether a drug changes the dopamine concentration released following a single pulse (1p) electrical stimulation.
Behavioral tests in rodents coupled with dopamine signaling manipulations
By onThese two protocols describe behavioral tests associated with Parkinson’s disease phenotypes in mice. The striatal motor learning protocol (rotarod test) is a two-phase paradigm assessing striatal motor learning under reversible dopamine antagonism.
Fast scan cyclic voltammetry
By onThis protocol describes the ex vivo fast-scan cyclic voltammetry (FSCV) technique for detecting dopamine in the dorsal striatum in mice.
Pull-down Assays
By onProtocol for protein pull-down assays as performed in "Synapsin E-domain is essential for α-synuclein function." (https://doi.org/10.7554/eLife.89687.1)
Hippocampal Cultures, lentivirus production, vGLUT1-pHluorin imaging and analysis
By onProcedures for hippocampal cultures, lentivirus production, vGLUT1-pHluorin imaging and analysis used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Proximity Ligation Assay (PLA)
By onProcedure for proximity ligation assay used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Immunofluorescence and object-based colocalization analysis
By onProcedures for immunofluorescence and object-based colocalization analysis used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Electron microscopy (EM) and quantification of inter-vesicular distances
By onProcedure for electron microscopy (EM) and quantification of inter-vesicular distances used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
αSyn Immunohistochemistry
By onProcedure for immunohistochemistry used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
