Very low-density lipoprotein receptor (VLDLR)-C-tag purification from HEK293E cells
By onThis protocol details how to purify recombinant Very low-density lipoprotein receptor (VLDLR)-C-tag protein from HEK293E cells.
SOP for cDNA synthesis by RT (Reverse Transcription) Promega kit
By onSOP for cDNA synthesis by RT (Reverse Transcription) Promega kit.
SOP for LBP ELISA after DSS-induced injury
By onA standard operating procedure (SOP) for performing LBP ELISA after inducing injury with DSS.
Quantitative PCR, 384 well format
By on384-well format is used in quantitative PCR to increase throughput, allowing simultaneous analysis of multiple samples in a high-throughput manner.
SOP for BCA protein assay and western immunoblotting
By onSOP for BCA protein assay and Western immunoblotting includes detailed steps for protein quantification and detection using specific reagents, equipment, and protocols to ensure accurate results in biochemical research.
SOP for TriZOL RNA and Protein Extraction
By onA Standard Operating Procedure (SOP) for extracting RNA and protein using TriZOL, a common reagent for isolating nucleic acids and proteins from biological samples.
SOP for Flow cytometry after DSS-induced gut and brain injury
By onStandard Operating Procedure (SOP) for conducting flow cytometry analysis on samples from mice with DSS-induced gut and brain injuries.
α-Synuclein aggregation monitored by thioflavin-T (ThT) fluorescence in a plate reader
By onThis protocol details how to efficiently monitor α-Synuclein aggregation by thioflavin T fluorescence in a plate reader.
Amyloid beta (Aβ) aggregates N-terminal labeling
By onThis protocol details how to efficiently label protein aggregates at the N-terminal using Amyloid beta as example.
Solid phase binding assay – Clusterin binding to Very Low-Density Lipoprotein Receptor (VLDLR)
By onThis protocol details how to monitor Clusterin binding to the Very Low-Density Lipoprotein Receptor (VLDLR) by Enzyme-linked immunosorbent assays (ELISA) adapted from Leeb et al. (2014).
Tau aggregation monitored by thioflavin-T (ThT) fluorescence in a plate reader
By onThis protocol details how to efficiently monitor Tau aggregation by thioflavin T fluorescence in a plate reader.
Clusterin cellular uptake assay
By onThis protocol details how to efficiently monitor Clusterin and Clusterin/Substrate uptake in different cell types, like HEK293T, iNeurons and iMicroglia.
N_terminal protein labeling
By onThis protocol details how to efficiently label a protein at the N-terminus using Clusterin protein as example.
Analysis of glycosphingolipids from cell lines
By onA sensitive method to analyze the full complement of GSL structures present in mammalian cells, fluids, and tissues.
DAB Staining for GFP on Free-floating Fixed NHP Brain Tissue
By onThis protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex to detect green fluorescent protein (GFP).
Standard DAB Staining for Free-floating Fixed NHP Brain Tissue
By onThis protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex.
Melatonin ELISA
By onMeasurement of mouse plasma melatonin using ELISA Kit (Enzo Life Sciences, ENZ-KIT150-0001, NY, US) according to manufacturer instructions.
In-vivo MRI and CT scanning protocol for non-human primates using a 3D printed MR-compatible stereotaxic frame
By onThis protocol describes the methods of using a custom 3D printed stereotaxic frame to acquire MRI and CT scans for nonhuman primates in detailed steps.
Total and ps129-aSyn levels with Western Blot
By onMeasure total and PS129 alpha-synuclein in mouse plasma.
