Preparation of an Enriched Synaptic Vesicle Fraction
By onProcedure for preparing enriched synaptic vesicle fractions used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Preparation of brain and Neuro2A lysates for biochemical assays and evaluation
By onProcedure for preparing brain and Neuro2A lysates for biochemical assays and evaluation used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
In vitro phosphorylation
By onProcedure for in vitro phosphorylation used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
In vitro dephosphorylation
By onProcedure for in vitro dephosphorylation used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Proteome analysis
By onProcedure for proteome analysis used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
By onProcedure for Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Proteomics: On-bead/in-solution digestion and phosphopeptide enrichment
By onProcedure for On-bead/in-solution digestion and phosphopeptide enrichment used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
ColabFold Protein Structure Prediction and Membrane Interaction Modeling
By onProcedure for ColabFold protein structure prediction and membrane interaction modeling used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Multi-electrode array (MEA)
By onProcedure for multi-electrode array used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Multiple Sequence Alignment
By onProcedure for multiple sequence alignment used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Assessing autophagy using the HaloTag-LC3B cleavage assay
By onAssay to detect HaloTag-LC3B during starvation autophagy based on https://doi.org/10.7554/eLife.78923.
Patient PBMC flow cytometry
By onFlow cytometry analysis of peripheral blood mononuclear cells (PBMC) from a patient was performed.
DQ-BSA and BMV109 microscopy
By onDQ-BSA and BMV109 microscopy used for studying peritoneal macrophages.
pH-Rodo pathogens to monitor phagocytosis in pMacs
By onpH-Rodo pathogens are used to track phagocytosis in peritoneal macrophages (pMacs).
Flow cytometry for ex vivo stimulated pMacs
By onFlow cytometry used to analyze ex vivo stimulated peritoneal macrophages for research purposes.
Ea(52-68) uptake assays
By onEa(52-68) uptake assays were performed on peritoneal macrophages (pMacs).
Evaluating GPNMB ACD mutants by Western Blotting and immunofluorescence
By onThis protocol describes the evaluation of cellular processing of GPNMB mutants by Western Blotting and Immunofluorescent imaging in a HEK293 overexpression model.
Neural activation of the GI tract
By onProtocol for neuronal activation of the enteric nervous system.
GCaMP6f Fluorescence in Ex Vivo Intestinal Preparation
By onProtocol for GCaMP6f imaging in ex vivo mouse intestinal tissue.
Tissue preparation, immunohistochemistry, Imaging, and Quantification
By onProtocol for neuronal imaging
