Tau aggregation monitored by thioflavin-T (ThT) fluorescence in a plate reader
By onThis protocol details how to efficiently monitor Tau aggregation by thioflavin T fluorescence in a plate reader.
α-Synuclein aggregation monitored by thioflavin-T (ThT) fluorescence in a plate reader
By onThis protocol details how to efficiently monitor α-Synuclein aggregation by thioflavin T fluorescence in a plate reader.
Analysis of glycosphingolipids from cell lines
By onA sensitive method to analyze the full complement of GSL structures present in mammalian cells, fluids, and tissues.
Standard DAB Staining for Free-floating Fixed NHP Brain Tissue
By onThis protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex.
Melatonin ELISA
By onMeasurement of mouse plasma melatonin using ELISA Kit (Enzo Life Sciences, ENZ-KIT150-0001, NY, US) according to manufacturer instructions.
DAB Staining for GFP on Free-floating Fixed NHP Brain Tissue
By onThis protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex to detect green fluorescent protein (GFP).
In-vivo MRI and CT scanning protocol for non-human primates using a 3D printed MR-compatible stereotaxic frame
By onThis protocol describes the methods of using a custom 3D printed stereotaxic frame to acquire MRI and CT scans for nonhuman primates in detailed steps.
Total and ps129-aSyn levels with Western Blot
By onMeasure total and PS129 alpha-synuclein in mouse plasma.
Analysis of glycosphingolipids from animal tissues
By onInterest in the role of cellular glycosphingolipids (GSLs) in health and disease led the authors to develop a sensitive method to analyze the full complement of GSL structures present in mammalian cells, fluids, and tissues.
Purification of Total RNA from Cells Using Spin Technology
By onTotal RNA can be efficiently purified from cells using spin technology, which involves binding RNA to a column, washing away contaminants, and eluting pure RNA for downstream applications.
v2 RNAscope
By onThis is a protocol for performing RNAscope® in situ hybridization analysis on fixed-frozen mouse brain tissue using the RNAscope® Multiplex Fluorescent v2 kit (Advanced Cell Diagnostics; ACD).
Cytotoxicity Assay Protocol
By onThis is the protocol for cytotoxicity assay in cancer cell lines using flow cytometry.
Descending Platform
By onBehavioral test to assess motor function in rodents using the descending inclined platform.
Rotarod (40 RPM and 2- RPM Docking)
By onMotor function behavioral test in mice using rotarod apparatus.
EEG Surgery- Telemetry implantation and recording
By onStandard Operating Procedure for EEG surgery including the preparation of EEG transponder (DSI), anesthesia and animal preparation, calibration, and recording.
Quantification of glutamate released from human induced pluripotent stem cells (iPSC) derived cortical neurons (CNs)
By onThis protocol describes the procedure to quantify glutamate released from induced pluripotent stem cells derived cortical neurons (iPSC-CNs) cultured in adherent monolayer.
Measuring dopamine release in human-derived iPSCs using High-Performance Liquid Chromatography (HPLC) coupled to Electrochemical Detection (ECD)
By onThis protocol allows for the detection of evoked or tonic dopamine release from human iPSC-derived dopamine neurons using High-Performance Liquid Chromatography (HPLC) coupled to electrochemical detection.
