Cross-modality frame alignment for surgical robot electrode insertion
By onThis protocol details step by step how to use stereotaxic frame features to align (or coregister) monkey head CT and MR images in a clinical ROSA ONE Brain robot in preparation for electrode insertion planning.
Electromyogram recordings for internal capsule stimulations in non-human primates
By onThis protocol outlines steps for performing EMG recordings to confirm implant location during internal capsule stimulation in monkeys. Supplementary to Liang et al. 2024 10.1016/j.jneumeth.2024.110133.
Assessment of implant accuracy using high-resolution postmortem MRI
By onThis protocol describes the steps to use postmortem imaging to assess the accuracy of an implant by visualizing implant location with a small thermal ablation and comparing its coordinates to image-based pre-surgical planning in nonhuman primates.
Western Blot in Mouse Brain Tissue for detecting pRab proteins
By onProtocol for detecting pRab proteins in mouse brain tissue.
Ventral Midbrain Genomic PCR
By onProtocol outlines ventral midbrain genomic PCR for research purposes.
Isothermal Titration Calorimetry of the Rubicon RH domain and Rab7
By on1:1 binding of Rubicon and Rab7.
Immunofluorescent Imaging and Analysis
By onFollowing treatment with antibodies/genetic methods of introducing fluorescent tags.
FSCV and Ephys Recording Setup
By onMethods detailing the recording setup using both electrophysiological (Ephys) and fast-scan cyclic voltammetry (FSCV) used by Amjad et al. 2024 10.1523/ENEURO.0001-24.2024.
In vivo Electrophysiology Protocol
By onProtocol outlines method for collecting extracellular single unit dopamine recordings.
DART Infusion Protocol
By onThe protocol outlines the procedure for DART (drug acutely restricted by tethering) intracranial infusion in mice
Electrophysiological recording from Brain Slices Protocol
By onProtocol for slice electrophysiological recordings with DART is outlined, providing step-by-step instructions.
VTA Surgery Protocol
By onProtocol outlines VTA surgery to infect dopamine neurons with virus, and implant cannulas & fibers intracranially in mice
Behavior Protocol
By onProtocol describes associative learning assay for testing extinction and conditioning behavior in mice.
Histology Protocol
By onThe protocol outlines the steps for histological processing of a mouse brain.
