In Vitro αS PFF Treatment
By onProtocol outlines preparation of alpha synuclein preformed fibrils for in vitro cell treatment to study protein uptake, processing, and clearance.
Lysosomal activity DQ-Red BSA assay
By onProtocol outlines DQ-Red BSA Assay for live cell treatment and fixation for imaging lysosomal activity.
Operant Behavior
By onThis protocol is for training mice operant conditioning to assess cognitive performance using the resources described in Matikainen-Ankney BA et al 2021.
Rotarod Test
By onBehavioral assay to measure motor impairments, specifically balance and coordination, in mice.
Open Field using Ethovision
By onProtocol for general locomotion in rodents using open field arenas and Ethovision video tracking. Purpose: Assess cognitive, spontaneous motor activity, and anxiety
Apomorphine-induced rotations
By onTo test for 6-OHDA lesion efficiency, rotational behaviors are assessed while mice are under the effects of apomorphine. This test is run during the dark phase of light cycle.
Bioluminescence Imaging
By onThis protocol describes a bioluminescence stepwise imaging protocol in mice with AAV-luciferase expression in the vagal afferents using the Ivis Lumina series III imaging system.
Isolation of brain infiltrating lymphocytes
By onThis protocol details the isolation of brain infiltrating lymphocytes after adoptive transfer of mitochondrial antigen- specific T cells.
Preparation of qPCR standards for AAV titering
By onqPCR standards for AAV titering- document annex to protocol "AAV Purification Protocol with Iodixanol gradient"
Mitochondrial Antigen Presentation (MitAP) to 2CZ CD8+ T cell hybridoma
By onThis protocol details the Mitochondrial Antigen Presentation (MitAP) to 2CZ CD8+ T cell hybridoma
Food intake behavior protocol
By onThis protocol describes the behavioral test to measure food intake in mice.
Photometry Acquisition in Freely Moving Mice
By onThis protocol describes the steps for acquiring photometry data for freely moving mice.
Ex vivo electrophysiology
By onProtocol outlines ex vivo electrophysiology in mouse brain slices with guidance for clozapine N-oxide (CNO) testing in DREADD-expressing neurons.
Validating Responsiveness of AAV-DIO-hM3Dq-DREADD
By onProtocol for validating behavioral response to IP injection of clozapine-n-oxide in DAT-IRES-Cre mice injected with AAV-DIO-hM3Dq-DREADD in midbrain.
Human post-mortem nuclei preparation and single-nucleus multiome DNA/RNA sequencing by ResolveOME
By onProtocol for isolating nuclei, immunostaining, and sorting for single-nucleus DNA/RNA-seq using ResolveOME method is described for post-mortem brain samples.
Startle induced negative geotaxis (SING)
By onThis protocol describes how to perform the Startle induced negative geotaxis (SING) assay. SING defects in parkinsonism fly models have previously been shown to be caused by dopaminergic impairments.
DAB Staining for Tyrosine Hydroxylase (TH) on Free-floating Fixed NHP Brain Tissue
By onThis protocol details immunohistochemical 3,3’-Diaminobenzidine (DAB) staining for Tyrosine Hydroxylase (TH) of free-floating fixed brain tissue sections using the avidin/biotin ABC complex and the Rabbit Polyclonal Anti-TH NB300-109 (Novus).
